Natural peptides have limited use as therapeutics because they are very susceptible to degradation by host and bacterial protease enzymes. Natural peptides have limited use as therapeutics because they are very susceptible to degradation by host and bacterial protease enzymes. D-enantiomeric peptides are effective in inhibiting biofilm growth and eradicating certain types of multiply antibiotic-resistant Gram-negative bacteria.

DFK is an all D-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity.

DJK-5 Citations

    1. Zhang, Tian, et al. Journal of Endodontics (2021).
    2. Alford, M. A., et al. Frontiers in Cellular and Infection Microbiology 2021, 11, 75.
    3. Kłodzińska, Sylvia N., et al. Nanomedicine: Nanotechnology, Biology and Medicine 20, 102022 (2019).
    4. Huang, Xiangya, et al. Journal of Endodontics 45.12 (2019): 1522-1528.
    5. Reffuveille, Fany, et al. Frontiers in Microbiology 9 (2018): 2865.
    6. Wang, Dan, et al. Bioactive Materials 3.4 (2018): 418-425.
    7. Pletzer, Daniel, Sarah C. Mansour, and Robert EW Hancock. PLoS Pathogens 14.6 (2018): e1007084.
    8. Haney, Evan F., et al. Biomolecules 8.2 (2018): 29.
    9. Pletzer, Daniel, et al. Frontiers in Microbiology 8 (2017): 1867.
    10. Zhang, Tian, et al. PloS One 11.11 (2016): e0166997.
    11. Mansour, Sarah C., et al. EBioMedicine 12 (2016): 219-226.
    12. Ribeiro, Suzana Meira, et al. Antimicrobial Agents and Chemotherapy 59.7 (2015): 3906-3912.
    13. De La Fuente-Núñez, César, et al. Chemistry & Biology 22.2 (2015): 196-205.

Citations for Peptides that Incorporate D-Enantiomers

Targeting and monitoring ovarian cancer invasion with an RNAi and peptide delivery system.

Hao, Liangliang, Natalie Boehnke, Susanna K. Elledge, Nour-Saïda Harzallah, Renee T. Zhao, Eva Cai, Yu-Xiong Feng et al. Proceedings of the National Academy of Sciences 121, no. 11 (2024): e2307802121.

The azido-modified biosensor peptide (sequence B(biotin)-eGvndneeGffsarK-(FAM)dGGPLGVRGKK-(N3)) was synthesized by CPC Scientific with >90% purity.

Published On: April 11th, 2024Categories: Citations, Click Peptides, D-Amino Acids, Dye-Labeled
Ionic liquid-mediated transdermal delivery of thrombosis-detecting nanosensors.

Bekdemir, Ahmet, Eden EL Tanner, Jesse Kirkpatrick, Ava P. Soleimany, Samir Mitragotri, and Sangeeta N. Bhatia Advanced Healthcare Materials (2022): 2102685.

Peptides [TDN-qFAM, C-(PEG)2-K(CPQ2)-GGGSRPfGG-5FAM] were obtained from Tufts University Peptide Core Facility or CPC Scientific, Inc (USA)

Published On: March 14th, 2022Categories: Citations, D-Amino Acids, Dye-Labeled, FRET Substrates, PEGylation
Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs.

Axiak-Bechtel, Sandra M., Stacey B. Leach, David G. Scholten, Jessica R. Newton-Northup, Brendan J. Johnson, H. E. Durham, Kenneth A. Gruber, and Michael F. Callahan. Pharmacology Research & Perspectives 9, no. 3 (2021): e00777.

TCMCB07 [a cyclic substituted melanocortin antagonist with the structure Ac- Nle- cyclo[Asp- Pro- DNal(2’)-Arg- Trp- Lys]- DVal- DPro- NH2] was manufactured by CPC Scientific Inc. under cGMP conditions. Active pharmaceutical ingredient was dissolved in milliQ water at 10 mg ml−1, sterile filtered [..]”

Published On: May 20th, 2021Categories: Citations, D-Amino Acids, GMP Peptide Manufacturing, Peptide Macrocycles, publications, Showcase Macrocycles, Unnatural Amino Acids
Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Alford, M. A.; Choi, K.-Y. G.; Trimble, M. J.; Masoudi, H.; Kalsi, P.; Pletzer, D.; Hancock, R. E., Frontiers in Cellular and Infection Microbiology 2021, 2021, 11, 75.

"Peptides IDR-1018 (VRLIVAVRIWRR-NH2) (Achtman et al., 2012), DJK-5 (all D-amino acids vqwrairvrvir-NH2) (de la Fuente-Nunez et al., 2015) and IDR-1002 (VQRWLIVWRIRK-NH2) (Nijnik et al., 2010), were synthesized by CPC Scientific [..]"

Published On: March 12th, 2021Categories: Antimicrobial Peptides, Citations, D-Amino Acids
Recovery of Oral In Vitro Biofilms after Exposure to Peptides and Chlorhexidine.

Zhang, T.; Xia, L.; Wang, Z.; Hancock, R. E.; Haapasalo, M., Journal of Endodontics 2021, 47 (3), 466-471.

"Peptides DJK-5 and 1018 were synthesized using solid-phase 9-fluorenylmethoxy carbonyl chemistry and purified to a purity of >95% using reverse-phase high-performance liquid chromatography by CPC Scientific (Sunnyvale, CA) as previously described [..]"

Published On: March 1st, 2021Categories: Antimicrobial Peptides, D-Amino Acids
CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosis.

Baba, Osamu, Andrew Elvington, Martyna Szpakowska, Deborah Sultan, Gyu Seong Heo, Xiaohui Zhang, Hannah Luehmann et al. bioRxiv (2020).

The FC131 peptide (cyclo[2-Nal-Gly-d-Tyr-NMe-d-Orn-Arg]) was synthesized by CPC Scientific (Sunnyvale, CA).

Published On: January 2nd, 2020Categories: Citations, D-Amino Acids, Peptide Macrocycles, Unnatural Amino Acids
Non-invasive early detection of acute transplant rejection via nanosensors of granzyme B activity.

Mac, Quoc D., Dave V. Mathews, Justin A. Kahla, Claire M. Stoffers, Olivia M. Delmas, Brandon Alexander Holt, Andrew B. Adams, and Gabriel A. Kwong. Nature Biomedical Engineering 3, no. 4 (2019): 281.

"FITC-labelled GzmB substrate peptides with internal quencher ((5-FAM)aIEFDSGK(CPQ2)kkc) were synthesized by CPC Scientific and used for all in vitro activity assays."

Published On: February 18th, 2019Categories: Citations, D-Amino Acids, Dye-Labeled, FRET Substrates
PET-based Imaging of Chemokine Receptor 2 in Experimental and Disease-related Lung Inflammation.

Liu, Yongjian, et al. Radiology (2017): 161409.

"Synthesis, Labeling, and Stability of ECL1i. The ECL1i peptide (LGTFLKC) was synthesized from d-form amino acids by CPC Scientific (Sunnyvale, Calif)."

Published On: January 3rd, 2017Categories: Citations, D-Amino Acids, Metal Chelates
Magnetite biomineralization in Magnetospirillum magneticum is regulated by a switch-like behavior in the HtrA protease MamE.

Hershey, David M., et al. BioRxiv (2016): 047555.

"A custom peptidewith the sequence 5-carboxymethylfluorescein-Thr-Gln-Thr-Val-Ala-Ala-Gly-Ser-Lys(CPQ2)-D-Arg-D-Arg was obtainedcommercially (CPC Scientific)"

Published On: August 19th, 2016Categories: Citations, D-Amino Acids, Dye-Labeled, FRET Substrates
Design and NMR Studies of Cyclic Peptides Targeting the N-Terminal Domain of the Protein Tyrosine Phosphatase YopH.

Leone, Marilisa, et al. Chemical Biology & Drug Design 77.1 (2011): 12-19.

"Cyclo-DEYDDPfK; Cyclo-DE(pY)LDPfK; Cyclo-DE(FCOOH)LDPfK (FCOOH= phenylalanine with a carboxyl group at the para position) were purchased either from the MCW facility of the Wisconsin Medical College or from CPC Scientific (San José, CA, USA)"

Published On: November 30th, 2010Categories: Citations, D-Amino Acids, Peptide Macrocycles, Phosphorylated Peptides, Showcase Phosphorylation