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      1. Home
      2. Posts
      3. Phosphorylated Peptides

      Phosphorylated Peptides Archive

      • PRMT5 C-terminal Phosphorylation Modulates a 14-3-3/PDZ Interaction Switch.

        Espejo, Alexsandra B., et al. Journal of Biological Chemistry 292.6 (2017): 2255-2265.

        "PRMT5pT634 (Biotin-SAIHNPTGRSYpTIGL-COOH, where pT represents phosphothreonine), PGHS2 (Biotin-SGSGVLIKRRSTEL-COOH),PGHS2pT602 (Biotin-SGSGVLIKRRSpTEL-COOH), IRK1 (Biotin-SGSGPRPLRRESEI-COOH), IRK1pS425 (BiotinSGSGPRPLRREpSEI-COOH, where pS represents phosphoserine), ERBB4 (Biotin-GTVLPPPPYRHRNTVV-COOH), and ERBB4pT1306 (Biotin-GTVLPPPPYRHRNpTVV-COOH)) and by CPC Scientific, Inc. (E6 (HPV16) (Biotin-RSSRTRRETQLCOOH) and E6 (HPV16)pT156 (Biotin-RSSRTRREpTQLCOOH))."

        Published On: September 24th, 2016Categories: Citations, Phosphorylated Peptides
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      • The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes.

        Skjevik, Åge Aleksander, et al. Journal of Molecular Biology 426.1 (2014): 150-168.

        "The peptides TH-(1-43) (MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIE) and THp-(1-43) (MPTPDATTPQAKGFRRAVS(PO3)2-ELDAKQAEAIMSPRFIGRRQSLIE) were synthesized by CPC Scientific (San Jose, CA) at approximately 90% purity, as seen by mass spectroscopy, and used without further purification."

        Published On: January 9th, 2014Categories: Citations, Long Sequences, Phosphorylated Peptides
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      • The peripheral binding of 14-3-3γ to membranes involves isoform-specific histidine residues.

        Bustad, Helene J., Lars Skjaerven, Ming Ying, Øyvind Halskau, Anne Baumann, David Rodriguez-Larrea, Miguel Costas, Jarl Underhaug, Jose M. Sanchez-Ruiz, and Aurora Martinez. PloS One 7, no. 11 (2012): e49671.

        "The peptides TH-(1-43), i.e. MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIE, and its Ser19-phosphorylated counterpart THp-(1-43), i.e. MPTPDATTPQAKGFRRAVS(PO3)ELDAKQAEAIMSPRFIGRRQSLIE, were synthesized by CPC Scientific (San Jose, CA, USA) at approx. 90% purity, as seen by mass spectroscopy."

        Published On: November 26th, 2012Categories: Citations, Phosphorylated Peptides
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      • A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation.

        Havukainen, Heli, et al. The Journal of Experimental Biology 215.11 (2012): 1837-1846.

        "[..] polyserine tract of AmVg and NvVg were synthesized by CPC Scientific (Sunnyvale, CA, USA) for NMR analysis. The synthesis was performed after isotope-labeling expression attempts in Escherichia coli were deemed unsuitable [..] The peptides had the following sequences: AmVg (residues 358–392): EKLKQDILNLRTDISTS(Sp)SS(15I)SSSEENDFWQPKPT AmVg (residues 336–385): R(15V)SKT(15A)MNSNQI(15V)SDNS(15L)-(15S)STEEK(15L)KQDI(15L)N(15L)RTDI(15S)S(15S)(Sp)S(15A)IS (15S)(15S)EEND. NvVg (residues 351–385): EHKHSDESTSE(Sp)FES(15I)ADNNDDSYFQRKPKLTEAP." NvVg (residues 335–372): RPNK(15L)N(15L)QRRHDHKS(15G)EHKHSDE(15S)S-(15S)E(Sp)FE(15A)I(15A)DNNDD.

        Published On: May 9th, 2012Categories: Citations, Isotope-Labeled Peptides, Phosphorylated Peptides
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      • Design and NMR Studies of Cyclic Peptides Targeting the N-Terminal Domain of the Protein Tyrosine Phosphatase YopH.

        Leone, Marilisa, et al. Chemical Biology & Drug Design 77.1 (2011): 12-19.

        "Cyclo-DEYDDPfK; Cyclo-DE(pY)LDPfK; Cyclo-DE(FCOOH)LDPfK (FCOOH= phenylalanine with a carboxyl group at the para position) were purchased either from the MCW facility of the Wisconsin Medical College or from CPC Scientific (San José, CA, USA)"

        Published On: November 30th, 2010Categories: Citations, D-Amino Acids, Peptide Macrocycles, Phosphorylated Peptides, Showcase Phosphorylation
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      • Transition metal complex cations as reagents for gas-phase transformation of multiply deprotonated polypeptides.

        Crizer, David M., Yu Xia, and Scott A. McLuckey. Journal of the American Society for Mass Spectrometry 20.9 (2009): 1718-1722.

        Phosphopeptide LPISASHpSpSKTR was synthesized by CPC Scientific (San Jose, CA, USA). Both peptides were diluted to a concentration of 20 μM in 49/49/2 (vol/vol/vol) methanol/water/ammonium hydroxide solution."

        Published On: September 5th, 2009Categories: Citations, Phosphorylated Peptides
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