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      1. Home
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      Citations Archive

      • Substrate-Specific Conformational Regulation of the Receptor Tyrosine Kinase VEGFR2 Catalytic Domain.

        Solowiej, J., Chen, J.H., Zou, H.Y., Grant, S.K. and Murray, B.W. ACS Chemical Biology 8, no. 5 (2013): 978-986.

        • Oncology Research Unit, Pfizer Worldwide Research and Development, 10777 Science Center Drive, San Diego, California 92121, United States.

        EGFR2(786−805) peptide was synthesized and purified to 98% purity (CPC Scientific). Activation loop peptides were synthesized to >90% purity (Pfizer, La Jolla): VEGFR2 (Ac-LARDIYKDPDYVR-K KDR-tide); cMet (Ac-LARDMYDKEYYSK, METtide; MET2 Ac-ARDMYDKEYYSVHN-K). The C-terminal cMet peptide (Ac-ARDMYDKEYYSVHNK) was synthesized (Pfizer, La Jolla) to >90% purity.

        Published On: February 26th, 2013Categories: Citations
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      • The peripheral binding of 14-3-3γ to membranes involves isoform-specific histidine residues.

        Bustad, Helene J., Lars Skjaerven, Ming Ying, Øyvind Halskau, Anne Baumann, David Rodriguez-Larrea, Miguel Costas, Jarl Underhaug, Jose M. Sanchez-Ruiz, and Aurora Martinez. PloS One 7, no. 11 (2012): e49671.

        "The peptides TH-(1-43), i.e. MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIE, and its Ser19-phosphorylated counterpart THp-(1-43), i.e. MPTPDATTPQAKGFRRAVS(PO3)ELDAKQAEAIMSPRFIGRRQSLIE, were synthesized by CPC Scientific (San Jose, CA, USA) at approx. 90% purity, as seen by mass spectroscopy."

        Published On: November 26th, 2012Categories: Citations, Phosphorylated Peptides
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      • Preparation and preliminary biological evaluation of n-Gluc-Lys([Al 18F]NOTA)-TOCA.

        F.-H. Guo, et al. Journal of Nuclear and Radiochemistry, 34(3), 157-165 (2012)

        Glucitol-Lys(NOTA)-DPhe-Cys-Tyr-DTrp-Lys-Thr-Cys-Thr-OH

        Published On: November 4th, 2012Categories: Citations, Metal Chelates, Peptide Glycosylation, Showcase Peptide Chelates
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      • Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse.

        Welsh, J. D., et al. Journal of Thrombosis and Haemostasis 10.11 (2012): 2344-2353.

        "Thrombin-sensitive peptide (ThS-P) azidoacetyl alanine-K(5FAM)GALVPRGSAGK(CPQ2) was custom synthesized (2143 MW, > 95% purity; CPC scientific, Sunnyale, CA, USA) and dissolved in DMSO (20 mm ThS-P)."

        Published On: September 15th, 2012Categories: Citations, Click Peptides, Dye-Labeled, FRET Substrates
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      • Quantification of circulating D-dimer by peptide immunoaffinity enrichment and tandem mass spectrometry.

        Wang, W., Walker, N.D., Zhu, L.J., Wu, W., Ge, L., Gutstein, D.E., Yates, N.A., Hendrickson, R.C., Ogletree, M.L., Cleary, M. and Opiteck. Analytical Chemistry 84.15 (2012): 6891-6898.

        • Departments of Molecular Biomarkers, Cardiovascular Diseases, and Clinical Pharmacology, Merck Research Laboratories, Rahway, New Jersey, United States.

        "Peptide Stable isotope–labeled internal standard peptide (Q 7 and K 15 reciprocally cross-linked dimer of LTIGEGQQHHL*GGAKQAGDV, where L* represents 13 C 6 , 15 N 1 -labeled leucine) was synthesized and analyzed for purity and amino acid content (CPC Scientific)"

        Published On: July 9th, 2012Categories: Citations, Isotope-Labeled Peptides, Peptide Macrocycles, Showcase Isotope-Labeled, Showcase Macrocycles
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      • Critical determinants of human α-defensin 5 activity against non-enveloped viruses.

        Gounder, Anshu P., et al. Journal of Biological Chemistry 287.29 (2012): 24554-24562.

        "..folded HD5 was generated from a synthesized 80% pure linear peptide (CPC Scientific, Sunnyvale, CA) by thiol disulfide reshuffling overnight at room temperature in the presence of 3 mM reduced and 0.3 mM oxidized glutathione, 2 M guanidine hydrochloride, and 0.25 M sodium bicarbonate, pH 8.3, at a peptide concentration of 0.25 mg/ml [..] All α-defensins were quantified by UV absorbance at 280 nm using calculated molar extinction coefficients (18)."

        Published On: May 25th, 2012Categories: Antimicrobial Peptides, Citations, Multiple Disulfide Bridges
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      • A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation.

        Havukainen, Heli, et al. The Journal of Experimental Biology 215.11 (2012): 1837-1846.

        "[..] polyserine tract of AmVg and NvVg were synthesized by CPC Scientific (Sunnyvale, CA, USA) for NMR analysis. The synthesis was performed after isotope-labeling expression attempts in Escherichia coli were deemed unsuitable [..] The peptides had the following sequences: AmVg (residues 358–392): EKLKQDILNLRTDISTS(Sp)SS(15I)SSSEENDFWQPKPT AmVg (residues 336–385): R(15V)SKT(15A)MNSNQI(15V)SDNS(15L)-(15S)STEEK(15L)KQDI(15L)N(15L)RTDI(15S)S(15S)(Sp)S(15A)IS (15S)(15S)EEND. NvVg (residues 351–385): EHKHSDESTSE(Sp)FES(15I)ADNNDDSYFQRKPKLTEAP." NvVg (residues 335–372): RPNK(15L)N(15L)QRRHDHKS(15G)EHKHSDE(15S)S-(15S)E(Sp)FE(15A)I(15A)DNNDD.

        Published On: May 9th, 2012Categories: Citations, Isotope-Labeled Peptides, Phosphorylated Peptides
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      • Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail.

        Dvir, Hay, et al. Proceedings of the National Academy of Sciences 109.18 (2012): 6916-6921.

        "The biotinylated synthetic LDLR peptide [Biotin-PEG8-SINFDNPVYQKT (CPC Scientific)] was captured to ≈20–50 RU, whereas ≈200 RU of the J.D. mutant peptide (Biotin-PEG8-SINFDNPVCQKT) was required for proper detection of ARH binding."

        Published On: May 1st, 2012Categories: Citations, PEGylation, Showcase PEGylation
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      • Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.

        Shojaei, Farbod, et al. Journal of Experimental & Clinical Cancer Research 31.1 (2012): 1.

        • Pfizer Global Research and Development, Department of Oncology, La Jolla, CA, USA

        "Epitope mapping studies were carried out using an overlapping series of synthetic peptides (CPC Scientific, CA) designed based on the primary sequence of OPN. Peptides corresponding to the region 143-172 of human OPN are listed below [..]"

        Published On: March 23rd, 2012Categories: Citations, Peptide Libraries
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      • Supercritical fluid chromatographic resolution of water soluble isomeric carboxyl/amine terminated peptides facilitated via mobile phase water and ion pair formation.

        Patel, M. A., F. Riley, M. Ashraf-Khorassani, and L. T. Taylor. Journal of Chromatography A 1233 (2012): 85-90.

        • Global Research & Development, Pfizer, Inc., Groton, CT 06340, USA

        Uncapped peptides containing both an amine and carboxyl terminus were synthesized by CPC Scientific Inc. (San Jose, CA) for this research. The linear isomeric peptides exhibited high water solubility (e.g. 1 mg/mL).

        Published On: February 1st, 2012Categories: Citations
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