CPC performs a very specialized pooling service for its customers. We can customize this to suit any customer’s needs, but we have a general protocol that is very effective at demonstrating the contents of a pool. First, subpools of 20 peptides are created. MS is run on these pools to display each peak for each peptide contained in the subpool. In most cases, the peptides are of a different enough mass to clearly point out each peak. However, if the masses of the two peptides are very similar, it may be difficult or impossible to discern. After all of the subpools have been created, they are then combined into a master pool. Finally, MS is performed on the master pool with the notion of identifying one peak from each individual subpool. In doing so, this demonstrates that each subpool and subsequently each peptide are present in the master pool.
