When producing peptide-antibodies, it is important to use a carrier protein to elicit a better response from the animal. Commonly used carrier proteins are BSA and KLH, with KLH preferred for its higher immunogenicity. Peptides coupled with BSA will generate antibodies against both the peptide and the BSA, which could result in incorrect ELISA readings. […]
The reported purity of a peptide is a percentage of the target sequence as determined by HPLC. The impurities found in a peptide will include deletion sequences, truncated sequences, and incompletely deprotected sequences. Peptide content can be determined in a few different ways depending on the sequence and is a measurement of the native peptide […]
All peptides are delivered by gross weight unless otherwise specified. Gross peptide weight is the weight of the peptide and peptide impurities as well as components such as salts, counter ions, residual solvents, and water. Net peptide, on the other hand, is a calculation of merely the peptide present. In certain experiments, this can be […]
Immunological grade and > 70% purity
- Antigen for antibody production
- ELISA standard for measuring titers of antibodies in antisera
- Competitive elution chromatography
- Peptide array production
80% purity
- Non-quantitative enzyme-substrate studies
- Phosphorylation assays
- Non-quantitative peptide blocking studies
- Coupling to resins for affinity purification
- Coating of tissue culture plates for cell attachment
- Protein electrophoresis applications
95% purity
- Standards for […]
Because peptides are hygroscopic, it is important to allow the peptide to warm to Romm temperature prior to opening. Warming in a desiccator or dry box is the best way to avoid water condensation forming on the peptide. Weigh out the prodcut quickly in a clean environment only the needed quantity to be used to […]
