Mac, Quoc D., Anirudh Sivakumar, Hathaichanok Phuengkham, Congmin Xu, James R. Bowen, Fang-Yi Su, Samuel Z. Stentz et al. Nature Biomedical Engineering (2022): 1-15.
"FITC-labelled GzmB substrate peptides with internal quencher ((5FAM)aIEFDSG(K-CPQ2)kkc) were synthesized by CPC Scientific and used for all in vitro activity assays. [..] Quenched fluorescent peptides for screening candidate substrates ((5FAM)-{substrate}-(K-DABCYL)-amide"
Lopez, Andrea, Denis E. Reyna, Nadege Gitego, Felix Kopp, Hua Zhou, Miguel A. Miranda-Roman, Lars Ulrik Nordstrøm et al. Nature Communications 13, no. 1 (2022): 1-18.
"Hydrocarbon-stapled peptide corresponding to the BH3 domain of BIM, FITC-BIM SAHBA2: FITC-βAla-EIWIAQELRS5IGDS5F’NAYYA-CONH2, where S5 represents the non-natural amino acid inserted for olefin metathesis, was synthesized, purified at >95% purity by CPC Scientific Inc."
Soleimany, Ava P., [..] Sangeeta N. Bhatia. Cancer Research 81, no. 1 (2021): 213-224.
All peptides were synthesized by CPC Scientific (Sunnyvale, CA) and reconstituted in dimethylformamide (DMF) unless otherwise specified. Sequences are provided in Supplementary Table S1.
Cazanave, S.C., Warren, A.D., Pacula, M., Touti, F., Zagorska, A., Gural, N., Huang, E.K., Sherman, S., Cheema, M., Ibarra, S. and Bates, J. Science Translational Medicine 13, no. 616 (2021): eabe8939.
All peptides were synthesized by CPC Scientific Inc. For fluorogenic assays, peptides were flanked with the fluorophore-quencher pair 5-FAM-CPQ2. For in vivo urine experiments, peptides were barcoded with Glu-Fib–derived and heavy isotope–labeled peptides. PEGylated peptides were pooled prepared before in vivo experiments and stored at 4°C in phosphate-buffered saline.
Zeming, K. K.; Lu, R.; Woo, K. L.; Sun, G.; Quek, K. Y.; Cheow, L. F.; Chen, C.-H.; Han, J.; Lim, S. L. Analytical Chemistry 2021.
The FRET substrates used in this project are: neutrophil elastase substrate ((Z-AAAA)2Rh110 Abs/Em:497/520 nm, Anaspec), Granzyme B substrate (5TAMRA-VGPDFGR-K(QSY7)-NH2 Abs/Em: 546/580, CPC scientific), and metalloproteinase substrate (QSY21-HGDQMAQKSK(Cy5)-NH2 Abs/Em:649/666 nm, CPC Scientific).
Chan, Leslie W., Melodi N. Anahtar, Ta-Hsuan Ong, Kelsey E. Hern, Roderick R. Kunz, and Sangeeta N. Bhatia. Nature Nanotechnology (2020): 1-9.
HFA-modifed peptides were synthesized by CPC Scientifc (>95% purity). Briefy, the peptide substrate, Ac-CKKK(Cy5)-PEG4-Nle(O-Bzl)-Met(O)2-Oic-Abu-OH, was synthesized on Fmoc-Abu-CTC resin via standard Fmoc solid phase peptide synthesis.
Belenkaya, S. V., A. A. Bondar, T. A. Kurgina, V. V. Elchaninov, A. Yu Bakulina, E. A. Rukhlova, O. I. Lavrik, A. A. Ilyichev, and D. N. Shcherbakov. Biochemistry (Moscow), 2020.
A synthetic peptide of the following composition was used as a substrate: Dabcyl-HPHPHLSFMAIPK (5-FAM) KK-NH2 (Dabcyl = 4-(dimethylaminoazo) benzene-4-carboxylic acid, 5-FAM = 5-carboxyfluorescein) (CPC Scientific, USA) containing the Chn-sensitive region of bovine κ-casein.
Hao, Liangliang, Renee T. Zhao, Chayanon Ngambenjawong, Heather E. Fleming, and Sangeeta Bhatia. bioRxiv (2020).
All peptides were chemically synthesized by CPC Scientific, Inc. [..] K(N3)-ANP-GPVPLSLVMGGC [..] 5FAM-GGf-Pip-KSGGGK(CPQ2)-PEG2-GC
Buss, Colin G., and Sangeeta N. Bhatia. Proceedings of the National Academy of Sciences (2020).
Tandem Peptides. Tandem peptides were purchased from CPC Scientific. Sequences are: mTP-TAMRA-LyP1 (Myr-GWTLNSAGYLLGKINLKALA-ALAKKILGGGGK(5TAMRA)-CGNKRTRGC (C-C bridge)); [..] PEGylated formulations of these peptides were synthesized as previously described (49). The sequences are: [..] mTP-PEG-ARAL (Myr-GWTLNSAGYLLGKINLKALA-ALAKKILC-PEG5K-GGGARALPSQRSR).
Kudryashev, Julia A., Lauren E. Waggoner, Hope T. Leng, Nicholas H. Mininni, and Ester J. Kwon. ACS Sensors (2020).
Calpain substrate peptide (QSY21-QEVYGAMP-K(Cy5)-PEG2-GC- NH2) was synthesized by CPC Scientific Inc. (Sunnyvale, CA …
