Huang, Q., Johnson, T.W., Bailey, S., Brooun, A., Bunker, K.D., Burke, B.J., Collins, M.R., Cook, A.S., Cui, J.J., Dack, K.N. and Deal, J.G. Journal of Medicinal Chemistry 57, no. 4 (2014): 1170-1187.
- La Jolla Laboratories, Pfizer Worldwide Research and Development, 10770 Science Center Drive, San Diego, California 92121
The reactions were conducted in 50-μL volumes in 96-well plates and contained 1.3 nM wild-type or 0.5 nM mutant ALK, 3 μM phosphoacceptor peptide (5′FAM-KKSRGDYMTMQIG-CONH2, synthesized by CPC Scientific, Sunnyvale, CA)..
Magee, T.V., Brown, M.F., Starr, J.T., Ackley, D.C., Abramite, J.A., Aubrecht, J., Butler, A., Crandon, J.L., Dib-Hajj, F., Flanagan, M.E. and Granskog, K. Journal of Medicinal Chemistry 56, no. 12 (2013): 5079-5093.
- Pfizer Worldwide Research & Development, Pfizer, Inc., Groton, Connecticut 06340, United States
- CPC Scientific, Hangzhou, P.R. China
Solowiej, J., Chen, J.H., Zou, H.Y., Grant, S.K. and Murray, B.W. ACS Chemical Biology 8, no. 5 (2013): 978-986.
- Oncology Research Unit, Pfizer Worldwide Research and Development, 10777 Science Center Drive, San Diego, California 92121, United States.
EGFR2(786−805) peptide was synthesized and purified to 98% purity (CPC Scientific). Activation loop peptides were synthesized to >90% purity (Pfizer, La Jolla): VEGFR2 (Ac-LARDIYKDPDYVR-K KDR-tide); cMet (Ac-LARDMYDKEYYSK, METtide; MET2 Ac-ARDMYDKEYYSVHN-K). The C-terminal cMet peptide (Ac-ARDMYDKEYYSVHNK) was synthesized (Pfizer, La Jolla) to >90% purity.
Shojaei, Farbod, et al. Journal of Experimental & Clinical Cancer Research 31.1 (2012): 1.
- Pfizer Global Research and Development, Department of Oncology, La Jolla, CA, USA
"Epitope mapping studies were carried out using an overlapping series of synthetic peptides (CPC Scientific, CA) designed based on the primary sequence of OPN. Peptides corresponding to the region 143-172 of human OPN are listed below [..]"
Patel, M. A., F. Riley, M. Ashraf-Khorassani, and L. T. Taylor. Journal of Chromatography A 1233 (2012): 85-90.
- Global Research & Development, Pfizer, Inc., Groton, CT 06340, USA
Uncapped peptides containing both an amine and carboxyl terminus were synthesized by CPC Scientific Inc. (San Jose, CA) for this research. The linear isomeric peptides exhibited high water solubility (e.g. 1 mg/mL).
Khodai, T., Chappell, D., Christy, C., Cockle, P., Eyles, J., Hammond, D., Gore, K., McCluskie, M.J., Evans, D.M., Lang, S. and Loudon, P.T. Clinical and Vaccine Immunology 18, no. 10 (2011): 1702-1709.
- Vaccines Research Unit, Pfizer Research and Development, Sandwich, United Kingdom
Peptides (CPC Scientific) representing selected sequences of gD and gB that had previously been demonstrated to contain T cell epitopes (data not shown) were synthesized as 15-mers, quality assessed, and formulated into pools representing each protein.
Pacholec, M., Bleasdale, J.E., Chrunyk, B., Cunningham, D., Flynn, D., Garofalo, R.S., Griffith, D., Griffor, M., Loulakis, P., Pabst, B. and Qiu, X.Journal of Biological Chemistry 285, no. 11 (2010): 8340-8351.
- Departments of Cardiovascular, Metabolic and Endocrine Diseases, and Structural Biology, Pfizer Global Research and Development, Groton, Connecticut 06340
The p53-derived peptides, TAMRA-p53 peptide and native p53 peptide, were custom-synthesized by CPC Scientific.
Solowiej, J., Bergqvist, S., McTigue, M.A., Marrone, T., Quenzer, T., Cobbs, M., Ryan, K., Kania, R.S., Diehl, W. and Murray, B.W. Biochemistry 48, no. 29 (2009): 7019-7031.
- Pfizer Global Research and Development, La Jolla, Pfizer Inc., 10777 Science Center Drive, San Diego, California 92121.
A peptide designed to encompass VEGFR2 residues 786-805 was synthesized and purified to 98% purity (CPC Scientific, San Jose, CA). Compounds were synthesized as described in patents: AG-13736 (35), AG-13974 (36), AG-28038 (35), and AG-28047 (37).
Solowiej, J., Thomson, J.A., Ryan, K., Luo, C., He, M., Lou, J. and Murray, B.W. Biochemistry 47, no. 8 (2008): 2617-2630.
- Pfizer Global Research and DeVelopment, La Jolla, Pfizer Inc., 10777 Science Center Drive, San Diego, California 92121.
Peptide 4 [Dabcyl-KTSAVLQSGFRKME-Edans], peptide 5 [SITSAVLQ-pNA], and peptide 6 [SITSAVLQ-pNP] were purchased from CPC Scientific (San Jose, CA) and had the following purities: >95%, >93%, and >90.1%, respectively.
