Alvarez, N., Adam, G.C., Howe, J.A., Sharma, V., Zimmerman, M.D., Dolgov, E., Rasheed, R., Nizar, F., Sahay, K., Nelson, A.M. and Park, S. Viruses 16, no. 7 (2024): 1158.
- Center for Discovery and Innovation, Hackensack Meridian Health, 111 Ideation Way, Nutley, NJ 07110, USA
- Merck & Co., Inc., Rahway, NJ 07065, USA
The enzymatic activity of recombinantly expressed 3CLPro enzymes from different coronaviruses was measured using the following synthetic quenched FRET peptide: CP488-ESATLQSGLRKAK- (CPQ2)-NH2 (CPC Scientific, San Jose, CA, USA).
Shurtleff, V.W., Layton, M.E., Parish, C.A., Perkins, J.J., Schreier, J.D., Wang, Y., Adam, G.C., Alvarez, N., Bahmanjah, S., Bahnck-Teets, C.M. and Boyce, C.W. Journal of Medicinal Chemistry 67, no. 5 (2024): 3935-3958.
- Center for Discovery and Innovation, Hackensack Meridian Health. 111 Ideation Way. Nutley, New Jersey 07110, United States.
- Merck & Co., Inc., Rahway, New Jersey 07065, United States.
The enzymatic activity of recombinantly expressed 3CLPro enzymes from different coronaviruses was measured using the following synthetic quenched FRET peptide: CP488-ESATLQSGLRKAK- (CPQ2)-NH2 (CPC Scientific, San Jose, CA.
Design-rules for stapled peptides with in vivo activity and their application to Mdm2/X antagonists.
Chandramohan, A., Josien, H., Yuen, T.Y., Duggal, R., Spiegelberg, D., Yan, L., Juang, Y.C.A., Ge, L., Aronica, P.G., Kaan, H.Y.K. and Lim, Y.H. Nature Communications 15, no. 1 (2024): 489.
- Merck & Co., Inc., Kenilworth, NJ 07033, USA.
- Merck & Co., Inc., Boston, MA 02115, USA
- Merck & Co., Inc., West Point, PA 19486, USA
- Genentech, South San Francisco, CA 94080, USA
We thank Evans (Chen) Ge, Mike (Dixin) Xue, and Simon (Junhua) Li at Chinese Peptide Company (CPC) for peptide synthesis support.
Libiger, O., Shaw, L.M., Watson, M.H., Nairn, A.C., Umaña, K.L., Biarnes, M.C., Canet‐Avilés, R.M., Jack Jr, C.R., Breton, Y.A., Cortes, L. and Chelsky, D. Alzheimer's & Dementia 17, no. 12 (2021): 1976-1987.
- Janssen Research and Development, 3210 Merryfield Row, San Diego, CA 92121, USA.
- Foundation for the National Institutes of Health, North Bethesda, Maryland, USA
- Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA
- Merck & Co., Inc., West Point, Pennsylvania, USA
Purified synthetic stable isotope (15N and 13C) labeled (SIL) peptides were from CPC Scientific (EDSLEAGLPLQVR [CMGA]; SLGVGFATR [FABPH]; VLEYLNQEK [SCG2]; and THLGEALAPLSK [VGF]); and JPT Peptide Technologies (TNYLYGK [NPTX2]).
Wang, X., Kurowski, S., Wu, W., Castriota, G.A., Zhou, X., Chu, L., Ellsworth, K.P., Chu, D., Edmondson, S., Ali, A. and Andre, P. Journal of Pharmacology and Experimental Therapeutics 360, no. 3 (2017): 476-483.
- Departments of Cardiometabolic Disease Biology, Discovery Pharmaceutical Sciences, In Vitro Pharmacology, and Discovery Chemistry, Merck Research Laboratories, Kenilworth, New Jersey.
- Lead Optimization Chemistry, Merck Research Laboratories, Rahway, New Jersey.
The 7-amido-4-thifluoromethylcoumarin (AFC) containing fluorescence substrate, CH3SO2-cyclohexyl-Gly-Gly-Arg-AFC (cyclohexyl-G-G-R-AFC) was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)..
Barbieri, Christopher M., et al. Journal of Pharmacology and Experimental Therapeutics 360.3 (2017): 466-475.
- In Vitro Pharmacology and Cardiometabolic Diseases, Merck & Co., Inc., Kenilworth, New Jersey
"The 7-amido-4-thifluoromethylcoumarin (AFC) containing fluorescence substrate, CH3SO2-cyclohexyl-Gly-Gly-Arg-AFC (cyclohexylG-G-R-AFC) was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)"
Chappell, D.L., Lee, A.Y., Bernstein, H.S., Lassman, M.E. and Laterza, O.F. Bioanalysis 8, no. 22 (2016): 2341-2349.
- Merck & Co., Inc. Rahway, NJ07065, USA
Lopez, A., Reyna, D.E., Gitego, N. et al. Co-targeting of BAX and BCL-XL proteins broadly overcomes resistance to apoptosis in cancer. Nat Commun 13, 1199 (2022).
- Cardio-metabolic Disease Biomarkers, Merck Research Laboratories, Kenilworth, NJ., Merck & Co., Inc.
"Glucagon synthetic peptide and heavy isotope–labeled internal standards (ISs) of oxyntomodulin and glucagon containing 2 [ 13 C 6 - 15 N]-labeled leucines and 1 [ 13 C 9 - 15 N]-labeled phenylalanine were synthesized by CPC Scientific.."
Zhou, X., Huang, C.H., Lao, J., Pocai, A., Forrest, G., Price, O., Roy, S., Kelley, D.E., Sullivan, K.A. and Forrest, M.J. Cardiovascular Diabetology 14 (2015): 1-10.
- Departments of Cardiometabolic Diseases and In Vivo Pharmacology, Merck Research Laboratories, 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA
GLP-1 was purchased from CPC Scientific (San Jose, California). MK-0626 was synthesized by Merck & Co., Inc.
Adam, G.C., Meng, J., Rizzo, J.M., Amoss, A., Lusen, J.W., Patel, A., Riley, D., Hunt, R., Zuck, P., Johnson, E.N. and Uebele, V.N. Journal of Biomolecular Screening 20, no. 2 (2015): 212-222.
- Screening and Protein Sciences, Merck Research Labs, North Wales, PA, USA
Plates were incubated for 210 min at 25 °C followed by quenching with 25 uL water with 2% formic acid containing 600 nM of the internal standard with the sequence KTEEISEVN[L-U7]-OH (CPC Scientific).
