Hershey, David M., et al. BioRxiv (2016): 047555.
"A custom peptidewith the sequence 5-carboxymethylfluorescein-Thr-Gln-Thr-Val-Ala-Ala-Gly-Ser-Lys(CPQ2)-D-Arg-D-Arg was obtainedcommercially (CPC Scientific)"
Dudani, Jaideep S., et al. ACS Nano 9.12 (2015): 11708-11717.
"...protease activity are focused on functionalizing synthetic peptide substrates with reporters that emit ... In vivo, veiled nanosensors are selectively activated at the ..... sized by CPC Scientific, Inc. (V1: Biotin-PEG(5 kDa)-(KFAM)-..."
Kwong, Gabriel A., et al. Proceedings of the National Academy of Sciences 112.41 (2015): 12627-12632.
"Cysteine-terminated peptides (Q1 = 5FAM-GGPLGVRGKK(CPQ-2)-PEG2-C, CPC Scientific.."
Zhu, Shu, et al. Blood 126.12 (2015): 1494-1502.
"The custom-made thrombin-sensitive peptide azidoacetyl-AK(5FAM)-GALVPRGSAGK(CPQ2)-NH2 was obtained from CPC Scientific (Sunnyvale, CA) for click reactions to anti-CD61, as previously described."
Miles, Linde A., et al. PloS One 10.6 (2015): e0129103.
"Peptides corresponding to the substrate sequences L/VP4.1 and non-hydrolysable linker (NHL) were synthesized between a 5-carboxyfluorescein (5-FAM) fluorophore/CPC Quencher (CPQ2) quencher pair (CPC Scientific). Additional lysines were added for increased solubility giving final peptide sequences of CPQ2-IVYELQGP-K(5FAM)-KK-NH2 and CPQ2-GGSGGS-K(5FAM)-KK-NH2 for L/VP4.1 and NHL, respectively. The identity and sequence of the L/VP4.1 and NHL peptides were confirmed by CPC Scientific via LC-MS and determined to be 96.7% and 95.1% pure, respectively."
Jensen, Jesper Langholm, et al. Journal of Dairy Science 98.5 (2015): 2853-2860.
"... Dabcyl-HPHPHLSFMAIPK(5-FAM)KK-NH 2 (98% purity; Figure 1) was purchased from CPC Scientific (Sunnyvale, CA). ..."
Pemovska, T., Johnson, E., Kontro, M., Repasky, G.A., Chen, J., Wells, P., Cronin, C.N., McTigue, M., Kallioniemi, O., Porkka, K. and Murray, B.W. Nature 519, no. 7541 (2015): 102-105.
- La Jolla Laboratories, Pfizer Worldwide Research & Development, San Diego, California 92121, USA.
The reactions were conducted in 50 ml volumes in 96-well plates and contained GST-tagged human-recombinant ABL1(T315I) kinase intracellular domain (1 nM), 3 mM phosphoacceptor peptide, 59 FAM- EAIYAAPFAKKK-OH (CPC Scientific, also known as ProfilerPro Peptide 2, Caliper Life Sciences), test compound (11-dose threefold serial dilutions, 2% DMSO final) or DMSO only, 1 mM dithiothreitol (DTT), 0.002% Tween-20 and 5 mM MgCl2 in 25 mM HEPES, pH 7.1
Vanderpool, D., Grimshaw, C.E., Lawson, J.D. and Ermolieff, J. Analytical Biochemistry 473 (2015): 46-52.
- Enzymology and Biophysical Chemistry Group, Takeda California, San Diego, CA 92121, USA
- Computational Sciences and Crystallography Group, Takeda California, San Diego, CA 92121, USA
The mobility shift assay substrate (5-FAM-IPTSPITTTYFFFKKK) was purchased from CPC Scientific (Sunnyvale, CA, USA).
Doron L, Coppenhagen-Glazer S, Ibrahim Y, Eini A, Naor R, Rosen G, Bachrach G. PLoS One. 2014 Oct 30;9(10):e111329.
"Fusolisin’s restriction specificity was verified using the FRET substrate CPQ2-Gly-Phe-Ile-Thr-Ala-Phe-Pro-Lys-(5FAM)-Arg-Arg-NH2 that was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)."
Warren, Andrew D., et al. Journal of the American Chemical Society 136.39 (2014): 13709-13714.
"Thrombin-sensitive reporter 1 (R1) was synthesized by CPC Scientific, with the sequence Biotin-PEG5kDa-Lys(5FAM)-Gly-Gly-DPhe-Pro-Arg-Ser-Gly-Gly-Gly-Cys, where PEG5kDa is 5 kDa poly(ethylene glycol)."
