Description

Suc-AAPF-pNA is a sensitive chromogenic substrate for human leukocyte cathepsin G that is not reactive with elastasecan. It can be cleaved by cathepsin G (Km = 1.7 mM), subtilisins, chymotrypsin (Km = 60 µM), chymase (Km = 4 mM), and cyclophilin. Release of p-nitroanilide is monitored at 405-410 nm. This substrate can be used for inhibitor screening and kinetic analysis.

SEQUENCE: Suc-Ala-Ala-Pro-Phe-pNA

ONE-LETTER SEQUENCE: Suc-AAPF-pNA

MOLECULAR FORMULA: C₃₀H₃₆N₆O₉

MOLECULAR WEIGHT: 624.65

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [70967-97-4]

SYNONYMS: N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide

RESEARCH AREA: Apoptosis

REFERENCES:
Nakajima, K., and Powers, J.C. The Journal of Biological Chemistry 254(10), 4027-4032 (1979).
Ermolieff, J., Boudier, C., Laine, A., et al. The Journal of Biological Chemistry 269(47), 29502-29508 (1994).
Nakakubo, H., Fukuyama, H., Nakajima, M., et al. Yeast 16(4), 315-323 (2000).

SKU(s): SUBS-052A, SUBS-052B, SUBS-052C, SUBS-052D

KEYWORDS: dye-labeled