Description

This peptide is a spectrophotometric substrate for a chymotrypsin-like serine protease encoded by a hepatitis C virus (HCV) and is promising target antiviral therapeutic development. Due to the low catalytic efficiency of HCV?s serine protease, this continuous spectrophotometric substrate is a ideal for both kinetic analysis and high-throughput screening. It is hydrolyzed by HCV protease under second-order rate kinetics with a constant (kcat/Km) of 80,000 ? 10,000 M^-1s^-1.

SEQUENCE: Ac-Asp-Thr-Glu-Asp- Val-Val-Pro-Nva-O-4-phenylazophenyl ester

ONE-LETTER SEQUENCE: Ac-DTEDVVP-Nva-O-4-phenylazophenyl ester

MOLECULAR FORMULA: C₅₁H₇₀N₁₀O₁₇

MOLECULAR WEIGHT: 1095.2

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Protein Kinases and Phosphatases

 

REFERENCES:
Zhang R, Beyer BM, Durkin J et al Anal Biochem 270:268-275 (1999)

SKU(s): SUBS-050A, SUBS-050B

KEYWORDS: