Description

Highly soluble fluorogenic matrix metalloproteinase (MMP) substrate with the following kcat/km values (at pH 7.6, 37°C): 619.0 M^-1s^-1 for MMP-2, 209.0 M^-1s^-1 for MMP-9, 40.0 M^-1s^-1 for MMP-3, 21.0 M^-1s^-1 for MMP-1. The fluorescence increase is measured using an wavelength of 340 nm and emission wavelength of 485 nm. The relative high emission wavelength of EDANS allows convenient measurement of MMP activity in complex biological media like synovial fluid and culture medium.

SEQUENCE: DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH2 (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Dabcyl-GABA-PQGL-Glu(EDANS)-AK-NH2

MOLECULAR FORMULA: C₆₃H₈₈N₁₆O₁₄S₁

MOLECULAR WEIGHT: 1325.56

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [193475-71-7]

SYNONYMS: TNO211, DABCYL-γ-Abu-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

RESEARCH AREA: Cancer

REFERENCES:
B. Beekman, et al., FEBS Lett, 390, 221 (1996)

SKU(s): MMPS-021A, MMPS-021B

KEYWORDS: dye-labeled, FRET Substrate