Description

One area of focus in peptides for delivery of macromolecular cargo into cells has been on highly cationic cell-penetrating peptides (CPPs) like for example the TAT sequence from HIV-1. In a recent flow cytometry screening assay, INF7TAT-A5K peptide was determined to have the highest activity: 68% knockout as compared with 99% knockout when using an electroporation protocol optimized for RNP delivery in T cells.

SEQUENCE: H-Gly-Leu-Phe-Glu-Lys-Ile-Glu-Gly-Phe-Ile-Glu-Asn-Gly-Trp-Glu-Gly-Met-Ile-Asp-Gly-Trp-Tyr-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-OH

ONE-LETTER SEQUENCE: GLFEKIEGFIENGWEGMIDGWYGYGRKKRRQRR

MOLECULAR FORMULA: C₁₈₂H₂₇₅N₅₅O₄₈S

MOLECULAR WEIGHT: 4033.59

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Immunomodulation

REFERENCES:
Foss, D.V., Muldoon, J.J., Nguyen, D.N. et al. Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes. Nat. Biomed. Eng (2023).
https://doi.org/10.1038/s41551-023-01032-2

Algayer, B., O’Brien, A., Momose, A., Murphy, D.J., Procopio, W., Tellers, D.M. and Tucker, T.J. Novel pH selective, highly lytic peptides based on a chimeric influenza hemagglutinin peptide/cell penetrating peptide motif. Molecules, 2019, 24(11), 2079.

SKU(s): IMMO-007A, IMMO-007B

KEYWORDS: delivery, CRISPR, Cas9, Cas12a, base editing, genome editing, CAR-T