Description

This fluorogenic (FRET) substrate containing an anthraniloyl group and a 3-nitrotyrosine as the resonance energy transfer donor/quencher pair, was developed for the assay of 2A protease from human rhinovirus (kcat/Km = 154 M^-1s^-1), which has been viewed as an important enzyme target for antiviral intervention.

SEQUENCE: H-Thr-Arg-Pro-Ile-Ile-Thr-Thr-m-nitro-Tyr-Gly-Pro-Ser-Asp-Lys(Abz)-Tyr-OH (trifluoroacetate salt)

ONE-LETTER SEQUENCE: TRPIITT-m-nitro-YGPSD-K(Abz)-Y

MOLECULAR FORMULA: C₈₀H₁₁₈N₂₀O₂₆

MOLECULAR WEIGHT: 1775.94

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [213135-91-2]

SYNONYMS:

RESEARCH AREA: Antimicrobial

REFERENCES:
Q.M.Wang et al., Arch. Biochem. Biophys., 356, 12 (1998)

SKU(s): HRV14-002A, HRV14-002B

KEYWORDS: dye-labeled, FRET substrate