Description

MCA-HQKLVFFA-K(DNP)-NH2 is a fluorogenic substrate for α-secretase. Upon cleavage by α-secretase, 7-methoxycoumarin-4-acetyl (MCA) is released and its fluorescence can be used to quantify α-secretase activity. MCA displays excitation and emission maxima at 328 and 420 nm, respectively.

SEQUENCE: MCA-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Lys(DNP)-NH2 (trifluoroacetate salt)

ONE-LETTER SEQUENCE: MCA-HQKLVFFA-K(DNP)-NH2

MOLECULAR FORMULA: C₇₃H₉₄N₁₆O₁₉

MOLECULAR WEIGHT: 1498.7

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER:

SYNONYMS:

RESEARCH AREA: Alzheimer’s Disease

REFERENCES:
M. Farzan, et al., Proc. Natl. Acad. Sci. USA 97, 9712 (2000)
T. Kowalewski, and D.M. Holtzman, Proc. Natl. Acad. Sci. USA 96, 3688 (1999)
U.S. Ladror, et al., J. Protein. Chem. 13, 357 (1994)

SKU(s): AMYD-112A, AMYD-112B

KEYWORDS: dye-labeled, FRET Substrate