Description

Very sensitive and internally quenched fluorescent substrate for endothelin-converting enzyme-1 (ECE-1), a membrane-bound zinc metallopeptidase that is related to neprilysin in amino acid sequence. The kcat/Km value for the hydrolysis by ECE-1 was 1.9 x 10^7 M^-1s^-1 and 1.7 x 10^6 M^-1s^-1 for the hydrolysis by neprilysin. For MMP-2, MMP-9 the kcat/Km values are in the range of 10^4 M^-1s^-1 whereas for MMP-1 there was no hydrolysis observed. This peptide was hydrolyzed rapidly by stromelysin 1 (MMP-3) with kcat/Km = 218000 M^-1s^-1 and very slowly by gelatinase B (MMP-9) (kcat/Km=10100 M^-1s^-1). There was no hydrolysis by MMP-1 and MMP-2 observed.

SEQUENCE: MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH (trifluoroacetate salt)

ONE-LETTER SEQUENCE: Mca-RPPGFSAF-Lys(Dnp)

MOLECULAR FORMULA: C₆₆H₈₁N₁₅O₁₉

MOLECULAR WEIGHT: 1388.46

STORAGE CONDITIONS: -20 ± 5 °C

CAS REGISTRY NUMBER: [323577-36-2]

SYNONYMS: Mca-(Ala⁷,Lys(Dnp)⁹)-Bradykinin

RESEARCH AREA: Alzheimer’s Disease

REFERENCES:

SKU(s): AMYD-111A, AMYD-111B

KEYWORDS: dye-labeled, FRET Substrate