Commonly Used Oligonucleotide Abbreviations
- DNA = A, C, G, T, 5MeC [for 5-Me-dC]
- RNA = rA, rG, rC, rU
- 2′ O-Methyl (2’-OMe) = mA, mG, mC, mU, m5MeC [for 2′-OMe, 5-Me C]
- 2ʹ-O-Methoxyethyl (2’-MOE) = moeA, moeG, moeC, moeU, moe5MeC, moe5MeU [for 2ʹ-moe 5-methyl cytidine and uridine]
- Locked (2’-LNA) = lnA, lnG, lnC, lnU, ln5MeC, ln5MeU [for locked RNA 5-methyl cytidine and uridine]
- 2′-F = fA, fG, fC, fU
- Backbone bases: Phosphodiester bases = (PO), Phosphorothioate = (PS), Phosphoroamidate = (PN)
Standard Testing
- Appearance Visual White to off-white powder
- Purity HPLC NLT 85% or NLT 90%; as requested
- Identity, MW ESI-MS +/- 0.05% [3Da for theoretical MW of 6,000]
| A Adenine | C Cytosine | T/U Uracil / Thymine | G Guanine |
|
|---|---|---|---|---|
| DNA | A | C | T | G |
| RNA | rA | rC | U | rG |
| 2'-OMe | mA | mC | mT/U | mG |
| 2'-MOE | moeA | moeC | moeT/U | moeG |
| 2'-cEt | cEtA | cEtC | cEtT/U | cEtG |
| Locked (2'-LNA) | lnA (or +A) | lnC (or +C) | lnT/U (or +T/U) | lnG (or +G) |
| 2'-F | fA | fC | fT/U | fG |
| PO backbone = Phosphodiester (Standard), no indication necessary. | A | C | T/U | G |
| PS backbone = phosphorothioate, indicated with an * in between bases. | *A | *C | *T/U | *G |
| PN backbone = phosphoramidate, indicated with an ^ in between bases. | ^A | ^C | ^T/U | ^G |