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      1. Home
      2. Posts
      3. FRET Substrates

      FRET Substrates Archive

      • Cysteine and Aspartyl Proteases Contribute to Protein Digestion in the Gut of Freshwater Planaria.

        Goupil, Louise S., et al. PLoS Negl Trop Dis 10.8 (2016): e0004893.

        "Protease activity in regurgitant from three replicate tanks were compared using a mixture of 7 internally quenched fluorescent substrates available from CPC Scientific, Sunnyvale, California (Table 1) [AMYD-112, AMYD-114, AMYD-109, MMPS-024, SUBS-017, AMYD-111, CASP-060]. These substrates were chosen based on their diverse sequence composition to enable detection of multiple protease classes."

        Published On: August 8th, 2016Categories: Citations, FRET Substrates
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      • Mathematical framework for activity-based cancer biomarkers.

        Kwong, Gabriel A., et al. Proceedings of the National Academy of Sciences 112.41 (2015): 12627-12632.

        "Cysteine-terminated peptides (Q1 = 5FAM-GGPLGVRGKK(CPQ-2)-PEG2-C, CPC Scientific.."

        Published On: September 28th, 2015Categories: Citations, Dye-Labeled, FRET Substrates, PEGylation
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      • FXIa and platelet polyphosphate as therapeutic targets during human blood clotting on collagen/tissue factor surfaces under flow.

        Zhu, Shu, et al. Blood 126.12 (2015): 1494-1502.

        "The custom-made thrombin-sensitive peptide azidoacetyl-AK(5FAM)-GALVPRGSAGK(CPQ2)-NH2 was obtained from CPC Scientific (Sunnyvale, CA) for click reactions to anti-CD61, as previously described."

        Published On: September 17th, 2015Categories: Citations, Click Peptides, Dye-Labeled, FRET Substrates, Showcase Click Chemistry, Showcase FRET Peptides, Unnatural Amino Acids
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      • Seneca Valley Virus 3C pro Substrate Optimization Yields Efficient Substrates for Use in Peptide-Prodrug Therapy.

        Miles, Linde A., et al. PloS One 10.6 (2015): e0129103.

        "Peptides corresponding to the substrate sequences L/VP4.1 and non-hydrolysable linker (NHL) were synthesized between a 5-carboxyfluorescein (5-FAM) fluorophore/CPC Quencher (CPQ2) quencher pair (CPC Scientific). Additional lysines were added for increased solubility giving final peptide sequences of CPQ2-IVYELQGP-K(5FAM)-KK-NH2 and CPQ2-GGSGGS-K(5FAM)-KK-NH2 for L/VP4.1 and NHL, respectively. The identity and sequence of the L/VP4.1 and NHL peptides were confirmed by CPC Scientific via LC-MS and determined to be 96.7% and 95.1% pure, respectively."

        Published On: June 12th, 2015Categories: Citations, Dye-Labeled, FRET Substrates
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      • The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay.

        Jensen, Jesper Langholm, et al. Journal of Dairy Science 98.5 (2015): 2853-2860.

        "... Dabcyl-HPHPHLSFMAIPK(5-FAM)KK-NH 2 (98% purity; Figure 1) was purchased from CPC Scientific (Sunnyvale, CA). ..."

        Published On: May 1st, 2015Categories: Citations, FRET Substrates
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      • Properties of Hemoglobin Decolorized with a Histidine-Specific Protease.

        Shi, Jing, et al. Journal of Food Science 80.6 (2015): E1202-E1208.

        "The cleavage preference of AGP was tested using an E(Edans)-AAXAAK-(Dabcyl)-NH 2 fluorescent substrate kit (CPC Scientific Inc., Calif., USA) with “A” representing alanine residues, “K” lysine residues, and “X” different individual amino acid residues as specified by their one ..."

        Published On: April 29th, 2015Categories: Citations, FRET Substrates
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      • Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease.

        Doron L, Coppenhagen-Glazer S, Ibrahim Y, Eini A, Naor R, Rosen G, Bachrach G. PLoS One. 2014 Oct 30;9(10):e111329.

        "Fusolisin’s restriction specificity was verified using the FRET substrate CPQ2-Gly-Phe-Ile-Thr-Ala-Phe-Pro-Lys-(5FAM)-Arg-Arg-NH2 that was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)."

        Published On: October 1st, 2014Categories: Citations, Dye-Labeled, FRET Substrates
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      • Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse.

        Welsh, J. D., et al. Journal of Thrombosis and Haemostasis 10.11 (2012): 2344-2353.

        "Thrombin-sensitive peptide (ThS-P) azidoacetyl alanine-K(5FAM)GALVPRGSAGK(CPQ2) was custom synthesized (2143 MW, > 95% purity; CPC scientific, Sunnyale, CA, USA) and dissolved in DMSO (20 mm ThS-P)."

        Published On: September 15th, 2012Categories: Citations, Click Peptides, Dye-Labeled, FRET Substrates
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      • Steady-State and Pre-Steady-State Kinetic Evaluation of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) 3CLpro Cysteine Protease:  Development of an Ion-Pair Model for Catalysis.

        Solowiej, J., Thomson, J.A., Ryan, K., Luo, C., He, M., Lou, J. and Murray, B.W. Biochemistry 47, no. 8 (2008): 2617-2630.

        • Pfizer Global Research and DeVelopment, La Jolla, Pfizer Inc., 10777 Science Center Drive, San Diego, California 92121.

        Peptide 4 [Dabcyl-KTSAVLQSGFRKME-Edans], peptide 5 [SITSAVLQ-pNA], and peptide 6 [SITSAVLQ-pNP] were purchased from CPC Scientific (San Jose, CA) and had the following purities: >95%, >93%, and >90.1%, respectively.

        Published On: February 1st, 2008Categories: Citations, COVID-19, Dye-Labeled, FRET Substrates
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