Martinez, R., Blasina, A., Hallin, J.F., Hu, W., Rymer, I., Fan, J., Hoffman, R.L., Murphy, S., Marx, M., Yanochko, G. and Trajkovic, D. PloS One 10, no. 9 (2015): e0138616.
- Oncology Research Unit, Pfizer Worldwide Research and Development, 10724 Science Center Drive, San Diego, CA, 92121
The 60 minute enzymatic reaction contained 5 nM Mps1 catalytic domain (aa 510–857), 60 μM (~Km-level) ATP, 5 μM phosphoacceptor peptide substrate (5FAM-RQRRSIDDTIDSTRLFE-NH2; CPC Scientific, Sunnyvale, CA), 10 mM MgCl2, 4% DMSO (with or without inhibitor), 1 mM DTT, 0.01% Tween-20, 50 mM HEPES (pH 7.5).
Zhu, Shu, et al. Blood 126.12 (2015): 1494-1502.
"The custom-made thrombin-sensitive peptide azidoacetyl-AK(5FAM)-GALVPRGSAGK(CPQ2)-NH2 was obtained from CPC Scientific (Sunnyvale, CA) for click reactions to anti-CD61, as previously described."
Miles, Linde A., et al. PloS One 10.6 (2015): e0129103.
"Peptides corresponding to the substrate sequences L/VP4.1 and non-hydrolysable linker (NHL) were synthesized between a 5-carboxyfluorescein (5-FAM) fluorophore/CPC Quencher (CPQ2) quencher pair (CPC Scientific). Additional lysines were added for increased solubility giving final peptide sequences of CPQ2-IVYELQGP-K(5FAM)-KK-NH2 and CPQ2-GGSGGS-K(5FAM)-KK-NH2 for L/VP4.1 and NHL, respectively. The identity and sequence of the L/VP4.1 and NHL peptides were confirmed by CPC Scientific via LC-MS and determined to be 96.7% and 95.1% pure, respectively."
Pemovska, T., Johnson, E., Kontro, M., Repasky, G.A., Chen, J., Wells, P., Cronin, C.N., McTigue, M., Kallioniemi, O., Porkka, K. and Murray, B.W. Nature 519, no. 7541 (2015): 102-105.
- La Jolla Laboratories, Pfizer Worldwide Research & Development, San Diego, California 92121, USA.
The reactions were conducted in 50 ml volumes in 96-well plates and contained GST-tagged human-recombinant ABL1(T315I) kinase intracellular domain (1 nM), 3 mM phosphoacceptor peptide, 59 FAM- EAIYAAPFAKKK-OH (CPC Scientific, also known as ProfilerPro Peptide 2, Caliper Life Sciences), test compound (11-dose threefold serial dilutions, 2% DMSO final) or DMSO only, 1 mM dithiothreitol (DTT), 0.002% Tween-20 and 5 mM MgCl2 in 25 mM HEPES, pH 7.1
Vanderpool, D., Grimshaw, C.E., Lawson, J.D. and Ermolieff, J. Analytical Biochemistry 473 (2015): 46-52.
- Enzymology and Biophysical Chemistry Group, Takeda California, San Diego, CA 92121, USA
- Computational Sciences and Crystallography Group, Takeda California, San Diego, CA 92121, USA
The mobility shift assay substrate (5-FAM-IPTSPITTTYFFFKKK) was purchased from CPC Scientific (Sunnyvale, CA, USA).
Doron L, Coppenhagen-Glazer S, Ibrahim Y, Eini A, Naor R, Rosen G, Bachrach G. PLoS One. 2014 Oct 30;9(10):e111329.
"Fusolisin’s restriction specificity was verified using the FRET substrate CPQ2-Gly-Phe-Ile-Thr-Ala-Phe-Pro-Lys-(5FAM)-Arg-Arg-NH2 that was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)."
Warren, Andrew D., et al. Journal of the American Chemical Society 136.39 (2014): 13709-13714.
"Thrombin-sensitive reporter 1 (R1) was synthesized by CPC Scientific, with the sequence Biotin-PEG5kDa-Lys(5FAM)-Gly-Gly-DPhe-Pro-Arg-Ser-Gly-Gly-Gly-Cys, where PEG5kDa is 5 kDa poly(ethylene glycol)."
Huang, Q., Johnson, T.W., Bailey, S., Brooun, A., Bunker, K.D., Burke, B.J., Collins, M.R., Cook, A.S., Cui, J.J., Dack, K.N. and Deal, J.G. Journal of Medicinal Chemistry 57, no. 4 (2014): 1170-1187.
- La Jolla Laboratories, Pfizer Worldwide Research and Development, 10770 Science Center Drive, San Diego, California 92121
The reactions were conducted in 50-μL volumes in 96-well plates and contained 1.3 nM wild-type or 0.5 nM mutant ALK, 3 μM phosphoacceptor peptide (5′FAM-KKSRGDYMTMQIG-CONH2, synthesized by CPC Scientific, Sunnyvale, CA)..
Xu, Y., Cai, T.Q., Castriota, G., Zhou, Y., Hoos, L., Jochnowitz, N., Loewrigkeit, C., Cook, J.A., Wickham, A., Metzger, J.M. and Ogletree, M.L. Thrombosis and Haemostasis 111, no. 04 (2014): 694-704.
- Departments of Thrombosis, Cardiometabolic Diseases and In Vivo Pharmacology, Merck Research Laboratories, Kenilworth, New Jersey, USA
CHG-GR-AFC (CPC Scientific, Sunnyvale, CA, USA) was used for rabbit FIXa and FXa, and human activated protein C.
Welsh, J. D., et al. Journal of Thrombosis and Haemostasis 10.11 (2012): 2344-2353.
"Thrombin-sensitive peptide (ThS-P) azidoacetyl alanine-K(5FAM)GALVPRGSAGK(CPQ2) was custom synthesized (2143 MW, > 95% purity; CPC scientific, Sunnyale, CA, USA) and dissolved in DMSO (20 mm ThS-P)."
