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      1. Home
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      Citations Archive

      • Comparison of a High-Throughput Mass Spectrometry Method and Radioactive Filter Binding to Assay the Protein Methyltransferase PRMT5

        Maegley, K.A., Krivacic, C., Bingham, P., Liu, W. and Brooun, A. ASSAY and Drug Development Technologies 13, no. 4 (2015): 235-240.

        • Oncology Research Unit and 2 Oncology Medicinal Chemistry, Pfizer World Wide Research and Development, San Diego, California.

        MS solvents were purchased from Sigma and Burdick & Jackson. Custom peptides were synthesized by CPC Scientific.

        Published On: June 12th, 2015Categories: Citations
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      • The function of the milk-clotting enzymes bovine and camel chymosin studied by a fluorescence resonance energy transfer assay.

        Jensen, Jesper Langholm, et al. Journal of Dairy Science 98.5 (2015): 2853-2860.

        "... Dabcyl-HPHPHLSFMAIPK(5-FAM)KK-NH 2 (98% purity; Figure 1) was purchased from CPC Scientific (Sunnyvale, CA). ..."

        Published On: May 1st, 2015Categories: Citations, FRET Substrates
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      • Properties of Hemoglobin Decolorized with a Histidine-Specific Protease.

        Shi, Jing, et al. Journal of Food Science 80.6 (2015): E1202-E1208.

        "The cleavage preference of AGP was tested using an E(Edans)-AAXAAK-(Dabcyl)-NH 2 fluorescent substrate kit (CPC Scientific Inc., Calif., USA) with “A” representing alanine residues, “K” lysine residues, and “X” different individual amino acid residues as specified by their one ..."

        Published On: April 29th, 2015Categories: Citations, FRET Substrates
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      • Targeted delivery of nucleic acids.

        Ren, Yin, and Sangeeta N. Bhatia. U.S. Patent No. 9,006,415. 14 Apr. 2015.

        "The tandem peptide library used in this work was synthesized via standard FMOC solid-phase peptide synthesis and purified by high-performance liquid chromatography at the MIT Biopolymers Core, Tufts University Core Facility or CPC Scientific, Inc."

        Published On: April 14th, 2015Categories: Citations, Peptide Libraries
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      • Acute hemodynamic and renal effects of glucagon-like peptide 1 analog and dipeptidyl peptidase-4 inhibitor in rats.

        Zhou, X., Huang, C.H., Lao, J., Pocai, A., Forrest, G., Price, O., Roy, S., Kelley, D.E., Sullivan, K.A. and Forrest, M.J. Cardiovascular Diabetology 14 (2015): 1-10.

        • Departments of Cardiometabolic Diseases and In Vivo Pharmacology, Merck Research Laboratories, 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA

        GLP-1 was purchased from CPC Scientific (San Jose, California). MK-0626 was synthesized by Merck & Co., Inc.

        Published On: March 7th, 2015Categories: Citations
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      • Axitinib effectively inhibits BCR-ABL1 (T315I) with a distinct binding conformation.

        Pemovska, T., Johnson, E., Kontro, M., Repasky, G.A., Chen, J., Wells, P., Cronin, C.N., McTigue, M., Kallioniemi, O., Porkka, K. and Murray, B.W. Nature 519, no. 7541 (2015): 102-105.

        • La Jolla Laboratories, Pfizer Worldwide Research & Development, San Diego, California 92121, USA.

        The reactions were conducted in 50 ml volumes in 96-well plates and contained GST-tagged human-recombinant ABL1(T315I) kinase intracellular domain (1 nM), 3 mM phosphoacceptor peptide, 59 FAM- EAIYAAPFAKKK-OH (CPC Scientific, also known as ProfilerPro Peptide 2, Caliper Life Sciences), test compound (11-dose threefold serial dilutions, 2% DMSO final) or DMSO only, 1 mM dithiothreitol (DTT), 0.002% Tween-20 and 5 mM MgCl2 in 25 mM HEPES, pH 7.1

        Published On: February 9th, 2015Categories: Citations, Dye-Labeled
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      • Alpha-defensin HD5 inhibits furin cleavage of human papillomavirus 16 L2 to block infection

        Wiens, Mayim E., and Jason G. Smith. "Alpha-defensin HD5 inhibits furin cleavage of human papillomavirus 16 L2 to block infection." Journal of Virology 89, no. 5 (2015): 2866-2874.

        "Folded HD5 was made from a synthesized 80% pure linearized peptide (CPC Scientific, Sunnyvale, CA) and purified by reverse-phase high-pressure liquid chromatography, and an HD5 derivative containing L-α-aminobutyric acid in place of cysteine (HD5 Abu) was chemically synthesized, as previously described.."

        Published On: February 5th, 2015Categories: Citations, Multiple Disulfide Bridges
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      • Residence time and kinetic efficiency analysis of extracellular signal-regulated kinase 2 inhibitors.

        Vanderpool, D., Grimshaw, C.E., Lawson, J.D. and Ermolieff, J. Analytical Biochemistry 473 (2015): 46-52.

        • Enzymology and Biophysical Chemistry Group, Takeda California, San Diego, CA 92121, USA
        • Computational Sciences and Crystallography Group, Takeda California, San Diego, CA 92121, USA

        The mobility shift assay substrate (5-FAM-IPTSPITTTYFFFKKK) was purchased from CPC Scientific (Sunnyvale, CA, USA).

        Published On: December 20th, 2014Categories: Citations, Dye-Labeled
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      • Use of high-throughput mass spectrometry to reduce false positives in protease uHTS screens.

        Adam, G.C., Meng, J., Rizzo, J.M., Amoss, A., Lusen, J.W., Patel, A., Riley, D., Hunt, R., Zuck, P., Johnson, E.N. and Uebele, V.N. Journal of Biomolecular Screening 20, no. 2 (2015): 212-222.

        • Screening and Protein Sciences, Merck Research Labs, North Wales, PA, USA

        Plates were incubated for 210 min at 25 °C followed by quenching with 25 uL water with 2% formic acid containing 600 nM of the internal standard with the sequence KTEEISEVN[L-U7]-OH (CPC Scientific).

        Published On: October 21st, 2014Categories: Citations, Isotope-Labeled Peptides
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      • Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease.

        Doron L, Coppenhagen-Glazer S, Ibrahim Y, Eini A, Naor R, Rosen G, Bachrach G. PLoS One. 2014 Oct 30;9(10):e111329.

        "Fusolisin’s restriction specificity was verified using the FRET substrate CPQ2-Gly-Phe-Ile-Thr-Ala-Phe-Pro-Lys-(5FAM)-Arg-Arg-NH2 that was custom synthesized by CPC Scientific (Sunnyvale, CA, USA)."

        Published On: October 1st, 2014Categories: Citations, Dye-Labeled, FRET Substrates
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